Host Species: HEK293 MW: Heavy Chain: 52 kDa, Light Chain: 26 kDa Tag(s): none Clonality: Monoclonal Isotype: IgG1 Description:Recombinant human antibody recognizing the CD3 binding region on immune cells. Synonym(s): cd3, cd-3, cluster of differentiation 3, cd3 Assay Conditions: IL-2 luciferase Jurkat reporter cells were incubated with anti-CD3 (BPS Cat. No 71274) in SF-RPMI for 24 hours. BPS luciferase reagent was added and luminescence was quantified. RLU = Relative Luminescence Unit normalized to wells with RPMI only. Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol Purification: Protein A affinity chromatography from HEK293 supernatants. Storage / Stability: Stable for at least 12 months at -80°C. Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 – 0.5%) at 4°C, stable for 2 weeks. Application(s): Useful for the detection of CD3 and binding to immune cells expressing CD3 on their surface and useful as a functional grade antibody for T cell activation and expansion. Reference(s): 1.Chatenoud, L., et al. PNAS. 1994; 91(1): 123-127.

2.Herold, K., et al., NEJM. 2002; 346: 1692-1698.

Scientific Category: Immunotherapy Product Type: Antibody Data shown is lot-specific. Contact us for specific information on other lots.